S. Ishiyamashigemoto et al., CLINICAL CHARACTERIZATION OF POLYMORPHISMS IN THE SULFONYLUREA-RECEPTOR-1 GENE IN JAPANESE SUBJECTS WITH TYPE-2 DIABETES-MELLITUS, Diabetic medicine, 15(10), 1998, pp. 826-829
To assess the association of polymorphisms at the sulphonylurea recept
or (SUR1) gene with the development of Type 2 diabetes mellitus, 456 s
ubjects, 236 with Type 2 diabetes and 220 non-diabetic controls, were
analysed for variants at exon 7, exon 22 and intron 24 of the SUR1 gen
e by the polymerase chain reaction and restriction fragment length pol
ymorphism. The T761T substitution in exon 22 of the SUR1 gene was not
found in either diabetic patients or non-diabetic controls. Both the e
xon 7 variant and the intron 24 variant were present in both groups at
similar frequencies. No significant association was seen between eith
er variant and obesity. Diabetic patients homozygous for the -3C allel
e of intron 24 had a higher ratio of positive family history than pati
ents homozygous for the -3T allele (p = 0.03). We conclude that these
polymorphisms are not major determinants of diabetes and obesity in th
e Japanese population. (C) 1998 John Wiley & Sons, Ltd.