ELECTROGENERATION OF BIOTINYLATED FUNCTIONALIZED POLYPYRROLES FOR THESIMPLE IMMOBILIZATION OF ENZYMES

A new biotin derivative functionalized by a pyrrole group has been syn thesized. This functionalization has allowed the preparation of the fi rst example of a poly(pyrrole-biotin) film by oxidative polymerization . In addition. the successful copolymerization of a polypyridinyl comp lex of ruthenium(II) containing pyrrole groups with the biotin derivat ive provided redox copolymers exhibiting specific adsorption sites. Th e resulting copolymer electrodes were applied io the immobilization of a biotinylated glucose oxidase through an avidin bridge. An avidin-co njugated alkaline phosphatase was also immobilized by direct specific adsorption onto the biotinylated polymer. The amperometric response of the resulting biosensors to glucose and phenylphosphate has been stud ied.