CHEMILUMINESCENCE FLOW-SENSING SYSTEM FOR HYDROGEN-PEROXIDE WITH IMMOBILIZED REAGENTS

A novel chemiluminescence (CL) sensing system for hydrogen peroxide co mbined with flow-injection analysis (FIA) is described in this paper. It is prepared by electrostatically immobilizing the analytical reagen ts, luminol and cobalt(II), on a strongly basic anion-exchange resin a nd a weakly acid cation-exchange resin, respectively. Hydrogen peroxid e is sensed by the CL reaction with luminol and cobalt(II) bleeding fr om the ion-exchange column with immobilized reagents by hydrolysis. Th e calibration graph is linear in the range 4 x 10(-8) to 1 x 10(-5) mo l l(-1), and the detection limit is 1.2 x 10(-8) mol l(-1) hydrogen pe roxide. A complete analysis could be performed in 1 min with a relativ e standard deviation of <5%. The system could be used for 50 h and has been applied successfully to the determination of hydrogen peroxide i n rainwater and glucose in serum by measuring the formation of hydroge n peroxide from a packed bed reactor with immobilized glucose oxidase. (C) 1998 Elsevier Science B.V. All rights reserved.