ACTIVITY GEL AND ACTIVITY BLOTTING METHODS FOR DETECTING DNA-MODIFYING (REPAIR) ENZYMES

Authors
SEKI S AKIYAMA K WATANABE S TSUTSUI K
Citation
S. Seki et al., ACTIVITY GEL AND ACTIVITY BLOTTING METHODS FOR DETECTING DNA-MODIFYING (REPAIR) ENZYMES, Journal of chromatography. Biomedical applications, 618(1-2), 1993, pp. 147-166
Citations number
96
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
618
Issue
1-2
Year of publication
1993
Pages
147 - 166
Database
ISI
SICI code
0378-4347(1993)618:1-2<147:AGAABM>2.0.ZU;2-2
Abstract
Zymographical methods (activity gel, overlay gel, activity blot and ac tivity blotting) for detecting DNA-modifying (repair) enzymes are revi ewed. Emphasis is put on the novel activity blotting method in which D NA repair enzymes electrophoresed on a gel are blotted and detected on a damaged DNA-fixed nylon membrane. Its practical procedures, includi ng a non-radioactive detection procedure, and representative results a re also described.