L. Izem et al., ELASMOBRANCH FISH (SCYLIORHINUS-CANICULA AND RAJA-CLAVATA) MUSCLE AMP-DEAMINASE - SUBSTRATE-SPECIFICITY AND EFFECT OF EDTA AND ADENYLATE ENERGY-CHARGE, Comparative biochemistry and physiology. B. Comparative biochemistry, 106(2), 1993, pp. 383-386
1. The AMP deaminases from skeletal muscles of dogfish and skate were
shown to be specific to 5'-AMP. Among several adenine nucleotide analo
gs, only dAMP was deaminated to an extent lower than 5%. 2. Similar to
vertebrates AMP deaminases, these enzymes were inhibited when incubat
ed in the presence of EDTA solutions. 3. The activity of the enzymes w
as regulated by adenylic energy charge variations, depending on the si
ze of the total adenine nucleotide pool. 4. The shape of the adenylate
energy charge response curves of the dogfish and skate muscle AMP dea
minases do not distinguish the two enzymes.