PURIFICATION AND CHARACTERIZATION OF AN NAD-MALIC ENZYME FROM BRADYRHIZOBIUM-JAPONICUM A1017

Authors
CHEN F OKABE Y OSANO K TAJIMA S
Citation
F. Chen et al., PURIFICATION AND CHARACTERIZATION OF AN NAD-MALIC ENZYME FROM BRADYRHIZOBIUM-JAPONICUM A1017, Applied and environmental microbiology (Print), 64(10), 1998, pp. 4073-4075
Citations number
21
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology (Print) → ACNP
ISSN journal
00992240
Volume
64
Issue
10
Year of publication
1998
Pages
4073 - 4075
Database
ISI
SICI code
0099-2240(1998)64:10<4073:PACOAN>2.0.ZU;2-2
Abstract
An NAD-malic enzyme was purified to homogeneity from Bradyrhizobium ja ponicum A1017, and its molecular characteristics were surveyed. The en zyme exhibited native and subunit molecular masses of 388 and 85 kDa, respectively, suggesting that it exists as a homotetramer, and was act ivated by metabolic intermediates in glycolysis. The role of the enzym e in bacteroids' carbon metabolism is discussed.