Px. Qi et al., THE BURST PHASE IN RIBONUCLEASE-A FOLDING AND SOLVENT DEPENDENCE OF THE UNFOLDED STATE, Nature structural biology, 5(10), 1998, pp. 882-884
Submillisecond hurst phase signals measured in kinetic protein folding
experiments have been widely interpreted in terms of the fast formati
on of productive folding intermediates. Experimental comparisons with
non-folding polypeptide chains show that, for ribonuclease A and cytoc
hrome c, these signals in fact reflect a shift from one biased ensembl
e of the unfolded state to another as a function of change in denatura
nt concentration.