INFLUENCE OF VINDESINE EXPOSURE ON THE MICRONUCLEUS FORMATION AND CELL-SURVIVAL IN V79 CELLS

Effect of different concentrations (0, 1, 5, 10, 20 and 50 nM) of vind esine sulphate was studied on clonogenicity and micronucleus (MN) form ation in V79 (Chinese hamster lung fibroblasts) cells. Exposure of V79 cells to vindesine for 6 h resulted in a concentration dependent decl ine in cell survival. The frequency of micronuclei (MN) increased in a concentration dependent manner at 16, 22 and 28 h post-exposure. The frequency of MN increased significantly after 5 to 50 nM drug exposure at 16 and 22 h post-treatment, while increasing post-exposure time to 28 h resulted in a significant increase in MN frequency at all exposu re doses of vindesine. The statistical evaluation of concurrent concen trations at various time periods showed a non-significant difference i n MN frequency among various post-exposure time periods, except 16 h a nd 28 h for 50 nM, where a significant decline in the MN frequency was observed at 28 h compared to 16 h post-exposure. The cell proliferati on indices showed a concentration dependent decline in the frequency o f binucleate cells and this decline was linear quadratic. The increasi ng drug concentration resulted in a concentration dependent decline in cell survival. While the frequency of micronuclei increased the cell survival decreased and the relationship between cell survival and micr onucleus induction was linear quadratic. (C) 1998 Elsevier Science B.V . All rights reserved.