PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST HUMAN BAD PROTEIN

More then 20 hybridoma cell lines secreting antibodies to recombinant human BAD protein were established. From five hybridomas monoclonal an tibodies were purified and characterised. Four monoclonal antibodies b elong to the IgG(1) subclass, One monoclonal antibody (MAb) (designate d as 6A11) belongs to the IgG(2b) subclass. All five MAbs have a speci ficity to different epitopes on the BAD protein. Four MAbs bound to th e BAD + Bcl-2 complex, and one antibody (designated as 5E6) bound only BAD. A sandwich enzyme immunoassay specific to the human BAD molecule was developed using the different MAbs, The results show that the obt ained antibodies are quite useful in studying the biological functions of BAD.