PRODUCTION AND CHARACTERIZATION OF A RABBIT MONOCLONAL-ANTIBODY AGAINST HUMAN CDC25C PHOSPHATASE

We produced a rabbit monoclonal antibody (MAb) against human CDC25C ph osphatase. The antibody reacts with a minimal epitope between amino ac ids 291-295 in the highly conserved C-terminal region of CDC25C. The a ntibody recognizes denatured CDC25C of recombinant and mammalian origi n in Western blot analysis. The corresponding rabbit polyclonal serum is able to immunoprecipitate the native protein, but this ability has been lost during the selection procedure. Although the production of t he rabbit MAb requires more effort and patience than the mouse MAb tec hnology, it offers a true alternative in case of antigens that are not immunogenic in mice.