AN IMMUNOENZYMATIC PROCEDURE FOR HUMAN APO B-CONTAINING PARTICLES QUANTIFICATION USING MONOCLONAL-ANTIBODIES

The present report describes a new monoclonal antibody-based enzyme im munoassay (ELISA) for the quantification of apolipoprotein (apo) B-con taining particles in plasma. Native low-density lipoprotein (LDL) and a reference serum were utilized to prepare the standard curve. Three d ifferent antibodies to apo B-100-4A6E3, 6A10B10, and 2D9-all produced in our laboratory, were examined. The apparent affinity constants of t he monoclonal antibodies (MAbs) 4A6E3, 6A10B10, and 2D9 were 2.9 x 10( 9), 1.74 x 10(9), and 0.63 x 10(8), respectively. The standard curve w as generated for an apo B-LDL range of 0.1 to 4.0 mu g/ml. Evaluating the concentration of apo B-containing particles in plasma may allow fo r a more accurate assessment of the risk of coronary artery disease.