A FAST NONFLUORESCENT STAINING METHOD FOR THE DETECTION OF PRIMED IN-SITU SYNTHESIS PRODUCTS

For the assessment of chromosomal numeral aberrations in cells, the me thod of choice is fluorescent in situ hybridisation (FISH) or, a newly introduced technique, oligonucleotide primed in situ hybridisation (P RINS). In the PRINS method labeled nucleotides are incorporated into n ewly synthesized DNA mediated through the Tag polymerase. Both PRINS a nd FISH reactions are visualized with a fluorescent light detection sy stem. We present a method whereby PRINS products can be reliably and r apidly visualized by a streptavidin DAB detection system.