A STUDY ON THE RELATIONSHIP BETWEEN ANTISENSE EGFR CDNA FRAGMENTS ANDNUCLEAR MATRIX PROTEINS IN GLIOBLASTOMA CELLS

The association of antisense epidermal growth factor receptor (EGFR) c DNA fragments with nuclear matrix from EGFR-antisense transfected glio blastoma cell lines U343 and U87 was investigated. A 1015 bp DNA fragm ent (primer I-II) was amplified in both genomic DNA and nuclear matrix -associated DNA (NM DNA) from EGFR-antisense transfected glioblastoma cell lines U343E and U87E. Two different DNA fragments (940 bp and 110 bp) were amplified by primer I-III in both genomic DNA and NM DNA of U343E, while one 110 bp PCR product was shown with the same primer in both genomic DNA and NM DNA of U87E only. After EGFR-antisense transfe ction, the binding property of the 110 bp DNA fragment (primer IV-V) t o nuclear matrix was not affected. Southwestern blotting demonstrated the presence of antisense EGFR cDNA binding nuclear matrix proteins. O ur findings demonstrate that not only EGFR DNA is associated with nucl ear matrix, but the transfected antisense EGFR cDNA also binds to nucl ear matrix proteins. The nuclear matrix is most likely involved in the replication and transcription of antisense EGFR cDNA or hybridisation with sense mRNA iii vitro.