Investigations into the effect of diet on modern human hair isotopic values

Carbon and nitrogen isotopic analysis of body tissues is one of the few tec hniques that can furnish quantitative information about the diet of archaeo logical humans. The study of the effects of various diets on modern human isotopic values c an help to refine palaeodietary theories, and such work also enables the te sting of palaeodietary theories independent of archaeological remains and i nterpretations. This report discusses the use of modern human hair as a sample material for isotopic analysis, The biogenic carbon and nitrogen isotopic signal is wel l preserved in hair, and the isotopic values of the keratin can be related to diet. We show that atmospheric and cosmetic contamination of hair kerati n does not appear to affect the measured isotopic values. In a small study of Oxford residents, we demonstrate that the magnitude of the nitrogen isotopic values of hair keratin reflects the proportion of ani mal protein consumed in the diet: omnivores and ovo-lacto-vegetarians have higher delta(15)N than vegans, There was an observed relationship between t he reported amount of animal protein eaten (either meat or secondary animal products) and the nitrogen isotopic values within the two groups of omnivo res and ovo-lacto-vegetarians, indicating that an increasing amount of anim al protein in the diet results in an increase in the delta(15)N of hair ker atin. This provides the first independent support for a long-held theory th at, for individuals within a single population, a diet high in meat equates to elevated nitrogen isotopic values in the body relative to others eating less animal protein. The implications of such results for the magnitude of the trophic level eff ect are discussed. Results presented here also permit a consideration of th e effects of a change of diet in the short and long term on hair keratin is otopic values. (C) 1999 Wiley-Liss, Inc.