Dr. Mack et al., Probiotics inhibit enteropathogenic E-coli adherence in vitro by inducing intestinal mucin gene expression, AM J P-GAST, 39(4), 1999, pp. G941-G950
Citations number
40
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
Probiotic agents, live microorganisms with beneficial effects for the host,
may offer an alternative to conventional antimicrobials in the treatment a
nd prevention of enteric infections. The probiotic agents Lactobacillus pla
ntarum 299v and Lactobacillus rhamnosus GG quantitatively inhibited the adh
erence of an attacking and effacing pathogenic Escherichia coli to HT-29 in
testinal epithelial cells but did not inhibit adherence to nonintestinal HE
p-2 cells. HT-29 cells were grown under conditions that induced high levels
of either MUC2 or MUC3 mRNA, but HEp-2 cells expressed only minimal levels
of MUC2 and no MUC3 mRNA. Media enriched for MUC2 and MUC3 mucin were adde
d exogenously to binding assays and were shown to be capable of inhibiting
enteropathogen adherence to HEp-2 cells. Incubation of L. plantarum 299v wi
th HT-29 cells increased MUC2 and MUC3 mRNA expression levels. From these i
n vitro studies, we propose the hypothesis that the ability of probiotic ag
ents to inhibit adherence of attaching and effacing organisms to intestinal
epithelial cells is mediated through their ability to increase expression
of MUC2 and MUC3 intestinal mucins.