Poly(methylene blue) modified electrode sensor for haemoglobin

Poly(methylene blue) modified electrodes have been prepared by potential cy cling from aqueous electrolyte solution containing methylene blue on glassy carbon substrates. They have been used successfully for the determination of haemoglobin via its electrooxidation at fixed potential using the batch injection analysis technique in which whole blood samples diluted with phos phate or acetate buffer are injected directly over the modified electrode i mmersed in inert electrolyte, Electrode response is fast, stable, and repro ducible. For blood from healthy donors, a good linear correlation was found between the results from the electrochemical injection method and the: con ventional clinical spectrophotometric measurement after cyanidation. The bl ood from potentially ill donors showed deviations from the Linear correlati on suggesting that this may be a good method for detecting haematological p roblems. (C) 1999 Elsevier Science B.V. All rights reserved.