Serum samples of patients with rheumatoid arthritis contain a specific autoantibody to "denatured" aldolase A in the osteoblast-like cell line, MG-63

Objective-To identify rheumatoid arthritis (RA) specific autoantibody and i ts antigen in the human osteoblast-like cell line, MG-63. Methods-MG-63 cell extract was subjected to western blotting by using RA an d normal serum samples as probes. The autoantigen was purified acid its N-t erminal sequence was determined by automated Edman degradation. The reactiv ity of denatured aldolase A was evaluated by immunoblotting. Screening by e nzyme linked immunosorbent assay (ELISA) using the autoantibody was perform ed. Results-40 kDa protein was found only in the RA serum samples and it was id entified as aldolase A. A polyclonal antibody for rabbit muscle aldolase A bound to the 40 kDa protein and reacted in preference with the denatured en zyme. Using ELISA for denatured rabbit aldolase A, the autoantibody was fou nd in approximately 10% of RA patients, whereas it was not found in the oth er arthropathy and healthy adults. Conclusion This 40 kDa anti-aldolase A autoantibody, which was identified o nly in serum samples of RA patients with severe bone erosion, could be rela ted to a certain event that induces RA specific joint destructions.