A double-selective tissue culture system for isolation of wild-type poliovirus from sewage applied in a long-term environmental surveillance

Authors
Manor, Y Handsher, R Halmut, T Neuman, M Abramovitz, B Mates, A Mendelson, E
Citation
Y. Manor et al., A double-selective tissue culture system for isolation of wild-type poliovirus from sewage applied in a long-term environmental surveillance, APPL ENVIR, 65(4), 1999, pp. 1794-1797
Citations number
29
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
4
Year of publication
1999
Pages
1794 - 1797
Database
ISI
SICI code
0099-2240(199904)65:4<1794:ADTCSF>2.0.ZU;2-Z
Abstract
We describe a simple, cost-efficient, double-selective method for isolation of wild type poliovirus from sewage samples containing vaccine polioviruse s and other enteroviruses, with a detection limit of 18 to 50 PFU per I to 2 liters of sewage. By this method we were able to process 1,700 sewage sam ples collected between 1991 and 1996, from which 10,472 plaques were isolat ed, 41 of them being identified as wild-type polioviruses.