Ey. Shin et al., H-Ras is a negative regulator of alpha(3)beta(1) integrin expression in ECV304 endothelial cells, BIOC BIOP R, 257(1), 1999, pp. 95-99
Citations number
32
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
We have examined the role of Ras in integrin expression in ECV304 endotheli
al cells. Among the integrins examined in stable ECV304 transfectants expre
ssing dominant active H-Ras (DAR-ECV), expression of alpha(3)beta(1) integr
in showed a prominent reduction in all the DAR-ECV clones when compared to
the parental ECV304 cells. This implies that H-Ras negatively regulates the
expression of alpha(3)beta(1) integrin in ECV304 cells. When treated with
inhibitors of the Ras downstream pathway (LY294002, PD98059, SB203580), the
expression of alpha(3)beta(1) integrin was up-regulated most significantly
by LY294002, suggesting that among the downstream pathways of Ras, phospha
tidylinositol 3-kinase is a major determinant. With the application of bloc
king antibody to alpha(3)beta(1) integrin (2 - 2 x 10(4) nM), migration of
ECV304 cells was enhanced to maximal (18%) at 20 nM. These results suggest
that migration of endothelial cells could be modulated by H-Ras via alterat
ion of the expression levels of alpha(3)beta(1) integrin. (C) 1999 Academic
Press.