K. Ito et al., Molecular cloning of a novel 130-kDa cytoplasmic protein, Ankhzn, containing ankyrin repeats hooked to a zinc finger motif, BIOC BIOP R, 257(1), 1999, pp. 206-213
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
A novel gene was trapped in mouse embryonic stem cells with a promoterless
gene trap vector. Fused transcripts were isolated from the embryos by rapid
amplification of cDNA ends, which were used for full-length cDNA cloning.
The protein predicted from the cDNA consisting of 7143 nucleotides comprise
s 1184 amino acids, which was confirmed by in vitro transcription/translati
on assaying An antibody against the synthesized peptide reacted with an app
roximate 130-kDa protein on SDS-PAGE. A search of available databases revea
led that this protein is a novel protein composed of 17 ankyrin repeats hoo
ked to a zinc finger motif, which we named Ankhzn. Ankhzn was observed on t
he endosomal membrane on immunoelectron microscopic analysis. Ankhzn belong
s to a new subgroup of double zinc finger proteins which may be involved in
vesicle or protein transport. Ankhzn mRNA and its protein were expressed u
biquitously from embryonic day 10.5 to adulthood. (C) Academic Press.