Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)

Authors
Kim, DU Roh, TY Lee, J Noh, JY Jang, YJ Hoe, KL Yoo, HS Choi, MU
Citation
Du. Kim et al., Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata), BBA-MOL C B, 1437(3), 1999, pp. 409-414
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
ISSN journal
13881981 → ACNP
Volume
1437
Issue
3
Year of publication
1999
Pages
409 - 414
Database
ISI
SICI code
1388-1981(19990325)1437:3<409:MCAFEO>2.0.ZU;2-S
Abstract
We cloned and expressed a full-length cDNA encoding a phospholipase D of ty pe alpha (PLD alpha) from cabbage. Analysis of the cDNA predicted an 812-am ino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PL D has 83% and 80% identity with Arabidopsis PLDa and castor bean PLD, respe ctively. Expression of this cDNA clone in E. coli shows a functional PLD ac tivity similar to that of the natural PLD. (C) 1999 Elsevier Science B.V. A ll rights reserved.