Voltammetric behavior of mammalian tumor cells and bioanalytical applications in cell metabolism

The voltammetric behavior of human mammalian cells was studied by choosing human leukemia cells (HL60) and human erythroleukemia cells (HEL). The volt ammetric response of the cells was found having relation with cell metaboli c viability in culture course. For example, the fluctuations of peak curren ts of HL60 were parallel with the nutrients replenished or not, which can r eflect cell health state; the voltammetric response of HL60 regulated by th e anti-metabolic drug SF-Uriacil (5F-U) in culture course behaved in a much decreased manner, by which a voltammetric method for evaluating cytotoxici ty is proposed. In this paper, the relation between HEL cell metabolism and the activation of receptor Mpl by its Ligand TPO was also studied. Moreove r, the mechanism of cell voltammetric behavior was discussed. (C) 1999 Else vier Science S.A. All rights reserved.