A. Azaola et al., Use of surface response methodology to describe biomass production of Bifidobacterium infantis in complex media, BIOTECH TEC, 13(2), 1999, pp. 93-95
An optimized medium containing Trypticasein, phytone, yeast extract and glu
cose is proposed to improve growth of bifidobacteria with high yeast extrac
t concentration and decreased amounts of Trypticasein and phytone. These ne
w growth media overcome nutritional limitations by the type and amount of a
mino acids contained in these sources and produced an increase from 1.8 to
more than 4 g cell mass l(-1).