A programme was created to semi-automatically analyse protozoal digitised i
mages. Principal Component Analysis technique was used for species identifi
cation. After data collection and mathematical treatment, a three-dimension
al representation was generated and several protozoa (Opercularia, Colpidiu
m, Tetrahymena, Prorodon, Glaucoma and Trachelophyllum) species could be po
sitively identified.