J. Teruya-feldstein et al., Differential chemokine expression in tissues involved by Hodgkin's disease: Direct correlation of eotaxin expression and tissue eosinophilia, BLOOD, 93(8), 1999, pp. 2463-2470
Hodgkin's disease (HD) is a lymphoid malignancy characterized by infrequent
malignant cells surrounded by abundant inflammatory cells. In this study,
we examined the potential contribution of chemokines to inflammatory cell r
ecruitment in different subtypes of HD. Chemokines are small proteins that
are active as chemoattractants and regulators of cell activation. We found
that HD tissues generally express higher levels of interferon-gamma-inducib
le protein-10 (IP-10), Mig. RANTES, macrophage inflammatory protein-1 alpha
(MIP-1 alpha). and eotaxin, but not macrophage-derived chemotactic factor
(MDC), than tissues from lymphoid hyperplasia (LH). Within HD subtypes, exp
ression of IP-10 and Mig was highest in the mixed cellularity (MC) subtype,
whereas expression of eotaxin and MDC was highest in the nodular sclerosis
(NS) subtype. A significant direct correlation was detected between eviden
ce of Epstein-Barr virus (EBV) infection in the neoplastic cells and levels
of expression of IP-10, RANTES, and MIP-1 alpha. Levels of eotaxin express
ion correlated directly with the extent of tissue eosinophilia. By immunohi
stochemistry, IP-10, Mig, and eotaxin proteins localized in the malignant R
eed-Sternberg (RS) cells and their variants, and to some surrounding inflam
matory cells. Eotaxin was also detected in fibroblasts and smooth muscle ce
lls of vessels. These results provide evidence of high level chemokine expr
ession in HD tissues and suggest that chemokines may play an important role
in the recruitment of inflammatory cell infiltrates into tissues involved
by HD. (C) 1999 by The American Society of Hematology.