Identification of differentially expressed genes in photo-period sensitivegenic male sterile rice by randomly amplified cDNAs using RAPD primers

The fertile and sterile young panicle representational populations were con structed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility transformation. Two populations were analyzed using cDNA-RAPD. The results showed that: ( i) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taki ng an advantage in avoiding false positive caused by conventional sampling method. ( ii) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but dif ferent staining of intensity on gel. ( iii) 33 primers amplified differenti al cDNA bands between fertile and sterile cDNA populations, and the ratio o f polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragm ents complicated.