D. Hamamdzic et al., Prolonged induction of IL-8 gene expression in a human fibroblast cell line infected with reovirus serotype 1 strain Lang, CLIN IMMUNO, 91(1), 1999, pp. 25-33
Viruses which infect mucosal surfaces commonly infect these particular anat
omical sites based on both the virion structure and the interaction of the
virus with a particular microenvironment. We infected a human lung epitheli
al cell line, a human gut epithelial cell line, and a human lung fibroblast
cell line with reovirus 1/L to explore how this natural isolate of both th
e lung and the gut may interact with mucosal surfaces. While reovirus infec
tion of the gut and lung epithelial cell lines was lytic, a chronic infecti
on was established in the human lung fibroblast cell line. All three cell l
ines also produced interleukin-8 (IL-8) after infection with reovirus in, a
nd IL-8 production was not dependent upon viral replication. A prolonged pr
oduction of IL-8 was observed in the chronically infected lung fibroblast c
ell line, suggesting that this mucosal population may be involved in the ge
neration of inflammatory responses after the resolution of the initial lyti
c infection of the epithelium. These studies provide an in vitro model syst
em for analyzing the interaction of reovirus 1/L with resident mucosal cell
populations. (C) 1999Academic Press.