C57BL/6 mice develop T-cell-mediated experimental autoimmune encephalomyeli
tis (EAE) after immunization with the neuroantigen myelin oligodendrocyte g
lycoprotein. (MOG). We immunized CD28-deficient C57BL/6 mice to determine t
he role of T cell costimulation in the immune response to MOG. CD28(-/-) mi
ce developed experimental autoimmune meningitis (EAM). EAM is a fatal, acut
e disease characterized by simultaneous weakness in all limbs, photophobia,
irritability, and spatial disorientation. Histologically, EAM consisted of
an infiltrate of myeloid, monocytic, and lymphocytic leukocytes within the
leptomeninges. In contrast, the brain parenchyma was unaffected. EAM was m
ediated by CD4(+) T cells since CD4 depletion prevented the disease. Upon r
echallenge, mice in which EAM was prevented by CD4(+) cell depletion develo
ped EAE not EAM. Therefore, the presence or absence of CD28 determines the
initial phenotype of the immune response to MOG. EAM, which develops in the
absence of CD28, is a unique experimental model for immune-mediated asepti
c meningitis. (C) 1999 Academic Press.