Bioremediation with microorganisms is an attractive alternative to conventi
onal techniques, such as incineration and chemical treatment, for disposing
of pollutants. Recent progress in molecular biology, microbiology, and gen
etics is providing the driving force towards engineering improved microbes
and enzymes for bioremediation. A number of genetic engineering approaches
have been developed in the past several years that have proven useful in in
troducing/evolving the desired properties for different biodegradative path
ways or enzymes. The initial excitement generated in this area should conti
nue to pave the way for rational or irrational design of microbes or enzyme
s with novel remedial properties.