Modifying secretion and post-translational processing in insect cells

Authors
Ailor, E Betenbaugh, MJ
Citation
E. Ailor et Mj. Betenbaugh, Modifying secretion and post-translational processing in insect cells, CURR OPIN B, 10(2), 1999, pp. 142-145
Citations number
44
Categorie Soggetti
Microbiology
Journal title
CURRENT OPINION IN BIOTECHNOLOGY
ISSN journal
09581669 → ACNP
Volume
10
Issue
2
Year of publication
1999
Pages
142 - 145
Database
ISI
SICI code
0958-1669(199904)10:2<142:MSAPPI>2.0.ZU;2-E
Abstract
The baculovirus-insect cell system is a valuable tool for the expression of heterologous proteins. Due to limitations in the intracellular processing environment, however, heterologous secreted and membrane proteins are often insoluble, poorly processed, or contain 'non-human' modifications. Recent attempts to modify the insect cell secretory pathway by overexpressing proc essing factors have demonstrated the potential to overcome these limitation s.