The role of cell adhesion molecules in Drosophila heart morphogenesis: Faint sausage, shotgun/DE-cadherin, and laminin A are required for discrete stages in heart development
Ta. Haag et al., The role of cell adhesion molecules in Drosophila heart morphogenesis: Faint sausage, shotgun/DE-cadherin, and laminin A are required for discrete stages in heart development, DEVELOP BIO, 208(1), 1999, pp. 56-69
Heart development in the Drosophila embryo starts with the specification of
cardiac precursors from the dorsal edge of the mesoderm through signaling
from the epidermis. Cardioblasts then become aligned in a single row of cel
ls that migrate dorsally. After contacting their contralateral counterparts
, cardioblasts undergo a cytoskeletal rearrangement and form a lumen. Its s
imple architecture and cellular composition makes the heart a good system t
o study mesodermal patterning, intergerm layer signaling, and the function
of cell adhesion molecules (CAMs) during morphogenesis. In this paper we fo
cus on three adhesion molecules, faint sausage (fas) shotgun/DE-cadherin (s
hg/DE-Cad), and laminin A (lam A), that are essential for heart development
, fas encodes an Ig-like CAM and is required for the correct number of card
ioblasts to become specified, as well as proper alignment of cardioblasts.
shg/DE-Cad is expressed and required at a later stage than fas; in embryos
lacking this gene, cardioblasts are specified normally and become aligned,
but do not form a lumen. Additionally, cardioblasts of shg mutant embryos s
how a redistribution of phosphotyrosine as well as a loss of Armadillo from
the membrane, indicating defects in cell polarity. The shg phenotype could
be phenocopied by applying EGTA or cytochalasin D, supporting the view tha
t Ca2+-dependent adhesion and the actin cytoskeleton are instrumental for h
eart lumen formation. As opposed to cell-cell adhesion, cell-substrate adhe
sion mechanisms are not required for heart morphogenesis, but only for main
tenance of the differentiated heart. Embryos lacking the lam A gene initial
ly developed a normal heart, but showed twists and breaks of cardioblasts a
t late embryonic stages. We discuss our findings in light of recent results
that elucidate the function of different adhesion systems in vertebrate he
art development, (C) 1999 Academic Press.