R. Giroti et Vk. Kashyap, Detection of the source of mislabeled biopsy tissue paraffin block and histopathological section on glass slide, DIAGN MOL P, 7(6), 1998, pp. 331-334
Citations number
26
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
The aim of this study was to identify the source of surgical biopsy tissue
in paraffin block and histochemically stained biopsy section on a slide sus
pected to be mislabeled. Commercially available polymerase chain reaction (
PCR)-based human lymphocyte antigen (HLA) DQA1 and Polymarker kits (Roche M
olecular Systems, Branchburg, NJ, U.S.A.). were used to generate DNA profil
es from biopsy tissue material in paraffin block and on histological slide,
and the reference blood sample was collected from the patient. The source
of biopsy tissue was detected by HLA DQA1 and polymarker profiling. Profile
s obtained from biopsy material were consistent with those obtained from re
ference blood sample. The PCR-based DNA profiling techniques can determine
the source of minute tissue in paraffin block and stained tissue sections o
n slides routinely prepared for diagnosis of carcinoma.