Nonliposomal approach - A study of preparation of egg albumin nanospheres containing amphotericin-B

The stability of liposomes after introduction into the body is presently be ing discussed and needs thorough understanding. Hence, as a nonliposomal ap proach, egg albumin nanospheres were prepared by the pH-coacervation method , and a preliminary study was carried out of the influence of process varia bles on the size and shape of nanospheres by changing the pH of the albumin solution, concentration of albumin solution, and volume of cross-linking a gent. The batch prepared with an albumin medium of pH 9, 2% concentration, and 100 mu l of 4% glutaraldehyde-ethanol solution was found to have a sphe rical uniform shape with an average size of 497.6 nm. The ideal batch was l oaded with the systemic antifungal drug amphotericin-B. Drug-loaded nanosph eres were evaluated to study their in vitro release. They were found to exh ibit a biphasic pattern with a cumulative percentage release of 97.7%.