B. Reichenpfader et al., Characterization of a highly variable short tandem repeat polymorphism at the D2S1242 locus, ELECTROPHOR, 20(3), 1999, pp. 514-517
We report the evaluation of short tandem repeat (STR) locus D2S1242 (GDB ID
G00-309-429) for forensic purposes, investigated by polymerase chain react
ion (PCR) amplification and both native and denaturating polyacrylamide gel
electrophoresis in 147 unrelated Austrians. No deviations from Hardy-Weinb
erg expectations were observed. The mean exclusion chance (MEC) was 0.669,
the discriminating power (DP) was 0.947, and the observed heterozygosity ra
te was 0.856. An allelic ladder consisting of eight sequenced alleles (141-
167 and 175 bp) was constructed. Sequence analysis revealed that the locus
comprised two repeat motifs varying in number between alleles GAAA and GAAG
. According to the number of tetranucleotide repeats the smallest allele wa
s designated as 10 and the largest allele as 18.