Two-dimensional electrophoresis of Cereus peruvianus (Cactaceae) callus tissue proteins

Two-dimensional electrophoresis of Cereus peruvianus callus tissues grown i n culture media containing two different 2,4-dichlorophenoxyacetic acid (2, 4-D) and kinetin combinations was used to identify minor differences in pol ypeptide composition of these cell clones. Altered expression during growth in the two 2,4-D and kinetin combinations was apparent for 13 polypeptides when calluses in the two media were compared. The number of proteins with differential expression (presence or absence of specific spots) was higher in callus tissues cultured in the 4.0 mg/L 2,4-D and 8.0 mg/L kinetin combi nation than in callus tissues cultured in the 4.0 mg/L 2,4-D and 4.0 mg/L k inetin combination. The present results show that the callus tissues mainta ined at 4.0 mg/L 2,4-D and 8.0 mg/L kinetin can be used as a matrix for in vitro selection programs.