Pathogenic, genetic and molecular characterisation of Fusarium oxysporum f.sp. lilii

Isolates of Fusarium oxysporum from lily were screened for pathogenicity, v egetative compatibility and DNA restriction fragment length polymorphisms, and compared to reference isolates of F. oxysporum f.sp. gladioli and F: ox ysporum f.sp. tulipae to justify the distinction of F: oxysporum f.sp. lili i. Twenty-four isolates from different locations in The Netherlands (18 iso lates), Italy (4 isolates), Poland and the United States (1 isolate each) s hared unique RFLP patterns with probes D4 and pFOM7, while hybridization di d not occur with a third probe (F9). Except for a self-incompatible isolate , these 24 isolates all belonged to a single vegetative compatibility group (VCG 0190). Isolates belonging to VCG 0190 were highly pathogenic to lily, but not to gladiolus or tulip, except for a single nonpathogenic isolate. Six saprophytic isolates of F: oxysporum from lily were nonpathogenic or on ly slightly aggressive to lily, gladiolus and tulip, belonged to unique VCG s and had distinct RFLP patterns. Three pathogenic isolates previously cons idered to belong to F: oxysporum f.sp. lilii were identified as F. prolifer atum var. minus all three belonged to the same VCG and shared unique RFLP p atterns. These three isolates were moderately pathogenic to lily and nonpat hogenic to gladiolus and tulip. The reference isolates of F. oxysporum f.sp . tulipae were pathogenic to tulip, but not to lily and gladiolus; they sha red a distinct RFLP pattern, different from those encountered among pathoge nic and saprophytic isolates from lily, and formed a separate new VCG (VCG 0230). Reference isolates of F. oxysporum f.sp. gladioli belonging to VCG 0 340 proved pathogenic to both gladiolus and lily, but not to tulip. These i solates, as well as isolates belonging to VCGs 0341, 0342 and 0343 of F. ox ysporum f.sp. gladioli, had RFLP patterns different from those encountered among the isolates from lily or tulip. These findings identify F: oxysporum f.sp. lilii as a single clonal lineage, distinct from F: oxysporum f.sp. g ladioli and f.sp. tulipae.