Computer-assisted sperm analysis technique was used in this study to object
ively evaluate sperm kinematics of 32 Garole ram semen ejaculates extended
@ 1:1 with egg yolk McIllvaine: glucose diluent initially, and subsequently
after 24 hr of storage at refrigeration temperature. Significant(P <0.01)
treatment effects were observed for motility and velocity traits. The mean
motility and rapidly motile spermatozoa for freshly diluted samples were 87
.8 % and 83.2 % as compared to 80.2% and 64.5 %, respectively, for stored s
amples. The respective curvilinear, average and straight line velocities fo
r freshly diluted and stored samples were 158.9, 130.6, 103.1 mu m/sec and
111.3, 91.2, 75 mu m/sec. Storage also had a significant (P <0.01) effect o
n mean amplitude of lateral head displacement and mean beat frequency, but
there was no significant (P >0.05) effect on per cent linearity and per cen
t straightness of spermatozoa. The results indicate the usefulness of this
technique for precisely evaluating the short-term preservability of Garole
ram semen.