Swab-based enzyme immunoassay system for detection of meat residues on food contact surfaces as a hygiene monitoring tool

An enzyme immunoassay system based on the use of a macroporous swab as a so lid phase for the capture and subsequent immunoenzymatic detection of immun oglobulin G (IgG) from meat residues on food contact surfaces was developed as a hygiene-monitoring tool. Moistened polyester swabs coated with anti-b ovine or anti-chicken IgG were rubbed on the test surface, and the captured IgG was subsequently detected directly on the swabs by brief sequential re actions with anti-bovine or anti-chicken IgG-peroxidase conjugate and chrom ogenic peroxidase substrate.