ENZYMES IN ORGANIC-SYNTHESIS .51. PROBING THE DIMENSIONS OF THE LARGEHYDROPHOBIC POCKET OF THE ACTIVE-SITE OF PIG-LIVER ESTERASE

Authors
PROVENCHER L WYNN H JONES JB KRAWCZYK AR
Citation
L. Provencher et al., ENZYMES IN ORGANIC-SYNTHESIS .51. PROBING THE DIMENSIONS OF THE LARGEHYDROPHOBIC POCKET OF THE ACTIVE-SITE OF PIG-LIVER ESTERASE, Tetrahedron : asymmetry, 4(9), 1993, pp. 2025-2040
Citations number
33
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Inorganic & Nuclear","Chemistry Physical
Journal title
Tetrahedron : asymmetry → ACNP
ISSN journal
09574166
Volume
4
Issue
9
Year of publication
1993
Pages
2025 - 2040
Database
ISI
SICI code
0957-4166(1993)4:9<2025:EIO.PT>2.0.ZU;2-1
Abstract
The dimensions of the large hydrophobic pocket (H(L)) of the active si te model of pig liver esterase (PLE) were probed using a series of ali phatic and phenylic malonates. Results from the hydrolyses of these ne w unnatural substrates permitted the extension of the H(L) pocket to g ive the new dimensions of 6.2 x 2.3 x 3.9 angstrom for a total volume of approximately 56 angstrom3.