Production and characterization of monoclonal antibodies specific to atrazine group compounds: Effects of coating ligand structure on the variation of sensitivity and specificity

Hybridoma cells were prepared by immunizing mice with carboxylic derivative s of atrazine conjugate to bovine serum albumin. After the screening of cul ture supernatant of hybridomas, five cell lines producing monoclonal antibo dies were established and 1.8-5.3 mi of ascitic fluid per mouse was obtaine d from each cell line. The protein A affinity purification yielded 0.35 - 0 .65 mg per ml of ascitic fluid from each cell line. The characterization st udies in terms of sensitivitiy and specificity indicate that MAb 2F9 and MA b 4B9 showed the best responses with atrazine and its group of ametryne and cyanazine, using microtiter plate coated with simazine derivative of 6-ami no hexanoic acid; no cross-reactivity was shown with simazine and cyanuric chloride.