The dependence for leukocyte function-associated antigen-1/ICAM-1 interactions in T cell activation cannot be overcome by expression of high density TCR ligand
C. Abraham et al., The dependence for leukocyte function-associated antigen-1/ICAM-1 interactions in T cell activation cannot be overcome by expression of high density TCR ligand, J IMMUNOL, 162(8), 1999, pp. 4399-4405
The leukocyte-specific integrin, LFA-1, can enhance T cell activation. Howe
ver, it is unclear whether the binding of LFA-1 to its ligand, ICAM-1, func
tions through intercellular adhesion alone, resulting in an augmentation of
the TCR signal, or involves an additional LFA-l-mediated cellular signal t
ransduction pathway. We have previously shown that naive CD4+ lymph node T
cells, isolated from DO11.10 TCR transgenic mice, are activated by increasi
ng doses of exogenous OVA peptide presented by transfectants expressing bot
h class II and ICAM-1, but not by cells expressing class II alone. To deter
mine whether LFA-1/ICAM-1 interactions were simply enhancing the presentati
on of low concentrations of specific MHC/peptide complexes generated from e
xogenously added peptide, we transfected cells with class II that is covale
ntly coupled to peptide, alone or in combination with ICAM-1. These cells e
xpress 100-fold more specific class II/peptide complexes than can be loaded
onto class II-positive cells at maximum concentrations of exogenous peptid
e. Despite this high density of TCR ligand, activation of naive CD4+ T cell
s still requires the coexpression of ICAM-1, LFA-1/ICAM-1 interactions are
not required for effective conjugate formation and TCR engagement because p
resentation of class II/peptide complexes in the absence of ICAM-1 does ind
uce up-regulation of CD25 and CD69, Thus, high numbers of engaged TCR canno
t compensate for the lack of LFA-1/ICAM-1 interactions in the activation of
naive CD4+ T cells.