A circulating bovine gamma delta T cell subset, which is found in large numbers in the spleen, accumulates inefficiently in an artificial site of inflammation: Correlation with lack of expression of E-selectin ligands and L-selectin

Tissue-specific localization of TCR-defined subsets of gamma delta T cells has been widely reported; however, the mechanisms responsible for this phen omenon are poorly understood. We describe a bovine gamma delta T cell TCR-a ssociated subset that preferentially localizes in the spleen, This subset w as characterized by coexpression of CD8, and was found to lack surface expr ession of E-selectin ligands, GR Ag ligands, as well as low expression of L -selectin, The CD8-positive gamma delta T cell subset did not accumulate at sites of inflammation as efficiently as CD8-negative gamma delta T cells t hat, in contrast, express E-selectin and GR ligands and high levels of L-se lectin, This is the first demonstration of a gamma delta T cell subset, whi ch exhibits a defined tissue tropism, having a unique adhesion molecule exp ression profile. These results demonstrate that in some cases tissue-specif ic accumulation of gamma delta T cell subsets can be predicted by expressio n, or lack of expression, of defined homing molecules.