Molecular analysis of the genetic variation among penicillin-susceptible and penicillin-resistant Streptococcus pneumoniae serotypes in Canada

Few studies have examined the genetic relatedness,within and between seroty pes of Streptococcus pneumoniae. Penicillin-susceptible S. pneumoniae (PSSP ) isolates (n = 170) and penicillin-resistant SI pneumoniae (PRSP) isolates (n = 48) belonging to 13 different serotypes from across Canada were studi ed to further understand the molecular epidemiology of S. pneumoniae. PRSP of serotypes 6B, 14, and 19F appeared to be novel clones,;differentiated by pulsed-field gel electrophoresis from the recognized international penicil lin-resistant clones belonging to these serotypes.:In addition, not all PRS P strains shared similar genetic backgrounds with PSSP strains of the same serotype,:suggesting that in Canada, certain PRSP strains have emerged from new unique lineages of PRSP. Although there was significant heterogeneity among S. pneumoniae of different serotypes, certain serotypes (3, 7F, and 2 2F) appeared to be more clonally related despite having originated from dif ferent geographic regions.