Application of molecular methods for detection and transmission analysis of Mycobacterium tuberculosis drug resistance in patients attending a reference hospital in Italy

A molecular analysis of drug-resistant isolates of Mycobacterium tuberculos is was done in a population with a high prevalence of human immunodeficienc y virus infection. Seventy-one consecutive isolates were tested for genotyp ic resistance to isoniazid, rifampicin, streptomycin, and ethambutol by pol ymerase chain reaction-single strand conformation polymorphism analysis and automated sequencing of target regions. Phenotypic and genotypic resistanc e to isoniazid, rifampicin, streptomycin, and ethambutol were detected in 2 3.4%, 11.2%, 7%, and 5.6% of isolates and in 87%, 88%, 40%, and 100% of res istant isolates, respectively. Specificity was 100% for all target regions. When rpoB, katG, and ahpC mutation analysis were combined, 86% of resistan t isolates to any drug were identified. No mutations in inhA were found in isoniazid-resistant isolates. Molecular detection of drug resistance, parti cularly for isoniazid and rifampicin, may represent a sensitive and very sp ecific technique. The strategy of selecting rpoB, katG, and ahp C to quickl y identify most resistant isolates, with a relevant saving of resources, is warranted.