Citation
L. Dorgan et al., Methods to determine biotin-binding capacity of streptavidin-coated magnetic particles, J MAGN MAGN, 194(1-3), 1999, pp. 69-75
Citations number
7
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
ISSN journal
03048853
→ ACNP
Volume
194
Issue
1-3
Year of publication
1999
Pages
69 - 75
Database
ISI
SICI code
0304-8853(199904)194:1-3<69:MTDBCO>2.0.ZU;2-K
Abstract
Two assays to determine the biotin-binding capacity of streptavidin magneti
c particles are described and compared. The two assays are based on the use
of biotinylated alkaline phosphatase and biotinylated fluorescein, respect
ively. Also, an assay for bound protein is presented. When the biotin-bindi
ng methods are combined with the protein assay, the specific activity can b
e determined. The fluorescent version is used to compare the streptavidin m
agnetic particles from several manufacturers. (C) 1999 Published by Elsevie
r Science B.V. All rights reserved.