Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

Authors
Matsunaga, T Sato, R Kamiya, S Tanaka, T Takeyama, H
Citation
T. Matsunaga et al., Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex, J MAGN MAGN, 194(1-3), 1999, pp. 126-131
Citations number
19
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
ISSN journal
03048853 → ACNP
Volume
194
Issue
1-3
Year of publication
1999
Pages
126 - 131
Database
ISI
SICI code
0304-8853(199904)194:1-3<126:CEIUPM>2.0.ZU;2-X
Abstract
Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemilumi nescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the lumin escence yield and the concentration of human IgG was obtained in the range of 1-10(3) ng/ml. (C) 1999 Published by Elsevier Science B.V. All rights re served.