Lysyl oxidase transcripts in peritoneal adhesions and incisional scars

OBJECTIVE: To evaluate the role of lysyl oxidase in postsurgical adhesion f ormation and incision wound repair. STUDY DESIGN: Female New Zealand rabbits underwent a pelvic-peritoneum adhe sion-inducing operation under sterile conditions. In brief, the uterine hor ns were removed from the abdomen and abraded with surgical gauze and a scal pel blade. The horns were then replaced into the abdominal cavity, the inci sion was sutured, and the animals were allowed to recover. The animals were killed before lesion development and after 2, 4, 8, and 14 days of postsur gical recovery. The abraded uterine horns, abdominal wall incisional wound and a portion of the sidewall peritoneum were then removed. Total RNA was e xtracted using the guanidinium thiocyanate-phenol-chloroform method. Northe rn blot analysis was performed with an [alpha-P-32]-labeled lysyl oxidase p robe. RESULTS: Lysyl oxidase was expressed during abdominal wall incision repair on days 2 and 4 postsurgical recovery, declining thereafter (days 8 and 14) . In contrast, no increase in lysyl oxidase expression was noted in the ute rine horns as compared to the control sidewall peritoneum. CONCLUSION: Lysyl oxidase plays a differential role in the early stages of abdominal wall and uterine horn repair.