Crystallization and preliminary X-ray analysis of active site-inhibited human coagulation factor VIIa (des-Gla)

Human coagulation factor VIIai that lacks the Gla domain (residues 1-44) ha s been prepared, purified, and crystallised, First, recombinant factor VII was activated to form factor VIIa, the active site was then inhibited with 1,5-dansyl-Glu-Gly-Arg-chloromethyl ketone, and finally the Gla domain was removed by chymotryptic digestion, yielding factor VIIai (des-Gla). After f urther purification single crystals suitable for x-ray analysis were obtain ed by vapour diffusion. Crystals of factor VIIai (des-Gla) belong to the te tragonal space group P4(1)2(1)2 or P4(3)2(1)2 with unit cell dimensions a = b = 94.85 Angstrom, c = 114.30 Angstrom contain one molecule per asymmetri c unit, and diffract to 2.3-Angstrom resolution when exposed to synchrotron radiation. (C) 1999 Academic Press.