Coexpression of potato PVYo coat protein and cryV-Bt genes in potato

The codon-modified cryV-Bt gene (cryV-Bt) from Bacillus thuringiensis subsp . kurstaki Berliner, which is specifically toxic to Lepidoptera and Coleopt era insects, and a potato virus Y degrees coat protein gene (PVY degrees cp ), in which the aphid transmission site was inactivated, were cotransformed into potato (Solanum tuberosum L.) 'Spunta' via Agrobacterium tumefaciens Conn. We demonstrated the integration and expression of both genes by molec ular analysis and bioassays, AllcryV-Bt/PVY degrees cp-transgenic lines mer e more resistant to potato tuber moth (Phthorimaea operculella Zeller) and PVY degrees infection than nontransgenic 'Spunta', Four cryV-Bt/PVY degrees cp transgenic lines were equal in potato tuber moth mortality to a cryV-Bt transgenic line, but of these four only two lines were equivalent in PVY d egrees titer levels to a PVY degrees cp-transgenic line, We identified two transgenic lines, 6a-3 and 6a-5,which showed greater resistance to potato t uber moth and PVY degrees than the other cuyV-Br/PVY degrees cp transgenic lines. This study indicated that multiple genes, conferring insect pest res istance and virus resistance, could be engineered into and expressed simult aneously in a potato cultivar.