G. Voggenreiter et al., Influence of immunosuppression on osteoinductive properties of demineralized bone matrix - Experimental studies in rats, LANG ARCH S, 1999, pp. 337-342
Background/Aim: The aim of the study was to evaluate the influence of immun
osuppression and reduction of cytokin synthesis on osteoinductive capacity
of demineralized bone matrix (DBM).
Methods: DBM was implanted in Lewis rats and the following groups were inve
stigated: autogeneic and xenogeneic DBM without immunosuppression (controls
) and autogeneic and xenogeneic DBM with immonosuppression by means of FK50
6 (1 mg/kg BW i,p per day). In-vitro cytokin synthesis (TNF-alpha), activit
y of alkaline phosphatase in the ossicle and histomorphometric analysis of
the induced bone and tibial metaphysis was performed.
Results: Treatment with the immunosuppressant FK506 significantly enhanced
bone induction in xenogeneic and non-immunogeneic isogeneic demineralized b
one matrix. In contrary marked bone resorption caused bone loss in the tibi
al metaphysis. Further on a reduced TNF-synthesis of whole blood was detect
ed.
Summary: We therefore propose positive and negative effects of FK506 on bon
e metabolism being independent from immunosuppression.